Journal: Vaccines

Article Title: Simultaneous Protective Immune Responses of Ducks against Duck Plague and Fowl Cholera by Recombinant Duck Enteritis Virus Vector Expressing Pasteurella multocida OmpH Gene

doi: 10.3390/vaccines10081358

Figure Lengend Snippet: Analysis of CD4+ ( A ) and CD8+ ( B ) T lymphocytes in peripheral blood using flow cytometry at 4 weeks and 8 weeks post-vaccination. Statistically significant differences are indicated by (***) p < 0.001, (**) p < 0.01, and (*) p < 0.05 compared with the PBS control.

Article Snippet: Then indirect staining of the cells was carried out as follows: mouse anti-duck CD8 alpha monoclonal antibody and mouse anti-duck CD4 monoclonal antibody (Bio-Rad) were added onto the cells and incubated for 30 min at 4 °C in the dark, and the cells were further stained with 1:200 diluted FITC-and PE-labelled goat anti-mouse IgG (Abcam, Cambridge, UK).

Techniques: Flow Cytometry, Control

Journal: Vaccines

Article Title: Simultaneous Protective Immune Responses of Ducks against Duck Plague and Fowl Cholera by Recombinant Duck Enteritis Virus Vector Expressing Pasteurella multocida OmpH Gene

doi: 10.3390/vaccines10081358

Figure Lengend Snippet: Levels of CD4+ and CD8+ response in survived ducks were analyzed at 7 days post-challenge with lethal DEV ( A ) and P. multocida X-73 ( B ) in different groups. Statistically significant differences are indicated by (***) p < 0.001, (**) p < 0.01 comparing the recombinant DEV-OmpHs with the DEV-WT or rOmpH group.

Article Snippet: Then indirect staining of the cells was carried out as follows: mouse anti-duck CD8 alpha monoclonal antibody and mouse anti-duck CD4 monoclonal antibody (Bio-Rad) were added onto the cells and incubated for 30 min at 4 °C in the dark, and the cells were further stained with 1:200 diluted FITC-and PE-labelled goat anti-mouse IgG (Abcam, Cambridge, UK).

Techniques: Recombinant

Journal: PLoS ONE

Article Title: Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck

doi: 10.1371/journal.pone.0271746

Figure Lengend Snippet: Spleen samples were collected from vaccinated duck at 21 dpv. The mRNA levels of cytokines (IL-2, IFN-α, IFN-γ, IL-6 and TNF-α) (A) and immune-related genes (MHC-I, MHC-II, CD4, CD8 and BAFF) (B) were analyzed by qPCR. Expression of target genes was normalized to those of duck β-Actin. The relative fold change was calculated using PBS injected duck as control. Data were indicated as mean ±SEM. Different letters (a~f) mean significant difference ( P <0.05). If there is one same letter, for example, ab means has no significant difference between adjacent a or b ( P >0.05).

Article Snippet: The duck spleen lymphocyte was separated and then incubated with mouse anti-duck CD4 or CD8 antibody (Bio-Rad, USA) 1:100 at 4°C for 1 h. After washing twice with washing liquid, FITC-Sheep anti-mouse/rat IgG (H+L) (Abcam, Cambridge, UK) secondary antibody 1:1000 was added and incubated in darkness for 1 h. Then washed twice and re-suspended in 4% paraformaldehyde.

Techniques: Expressing, Injection, Control

Journal: PLoS ONE

Article Title: Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck

doi: 10.1371/journal.pone.0271746

Figure Lengend Snippet: Splenic lymphocytes were isolated from the spleen of vaccinated duck at 21 dpv. Splenic lymphocyte was subjected to flow cytometry to assess the percentages of CD4 + (A) and CD8 + (B) T lymphocytes. (C) Data represent the mean ±SEM of three independent experiments. Different letters (a~c) mean significant difference ( P < 0.05).

Article Snippet: The duck spleen lymphocyte was separated and then incubated with mouse anti-duck CD4 or CD8 antibody (Bio-Rad, USA) 1:100 at 4°C for 1 h. After washing twice with washing liquid, FITC-Sheep anti-mouse/rat IgG (H+L) (Abcam, Cambridge, UK) secondary antibody 1:1000 was added and incubated in darkness for 1 h. Then washed twice and re-suspended in 4% paraformaldehyde.

Techniques: Isolation, Flow Cytometry